Effects of experimental protein-containing pneumococcal preparations on maturation of murine dendritic cells

Dendritic cells as the most active and highly specialized antigen presenting cells, play a key role in initiating immune responses. Currently, generation of medications activating dendritic for development anti-infective anticancer vaccines is relevance. Preparations microbial origin are promising to augment activity because they carry innate ligands Toll-like receptors. Such preparations include experimental protein-containing pneumococcal preparations, obtained from acetone-inactivated mass deposited S. pneumoniae 6B vaccine strain No. 296, followed by aqueous extraction separation 30—100 kDa fraction. were bone marrow CBA mice (n = 15), cultured complete growth medium RPMI-1640 added with recombinant GM-CSF IL-4 (Biosource, USA). On day 6, (50 pl/ml) administered immature cells. Commercial TNFa (20 ng/ml, Biosource, USA) was used standard maturation inducer (positive control). Immunophenotyping conducted using flow cytometry FITC- PE-labeled monoclonal antibodies against cell surface receptors: CD34, CD38, CD83, CD86, CD80, CD11c, MHC II, CD14, CD282 (TLR2), CD284 (TLR4), (eBioscience, Studying an effect on revealed that morphological characteristics generated did not differ significantly between each other well those induced TNFα. The characterized large sizes, oval or irregular shape, veiled cytoplasm, eccentrically located nucleus numerous long thin protrusions. Experimental proteincontaining decrease count CD34+ TLR2/TLR4+ increased expressing markers adhesion (CD38+), activation (MHC II+), costimulation (CD80/ CD86+) terminal differentiation (CD83+), which may evidence about events upon maturation. fraction molecules greater extent than extract more pronouncedly stimulated rise bearing costimulatory (p < 0.05). examined proteins regarding their CD83+ comparable. antigens derived shown induce precursors, TLR2 TLR4-expressing accounting effectors.